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crispick tool  (Broad Institute Inc)
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Broad Institute Inc crispick tool
Crispick Tool, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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crispick online tool  (Broad Institute Inc)
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Broad Institute Inc crispick online tool
a , b Heatmaps showing Pearson Correlations of clonal abundance across different tissues and organs. Each column corresponds to one primary recipient mouse. The clonal diversity of each mouse is shown below, where each color represents a unique clone. a Comparison between the peripheral blood, spleen, and bone marrow from the spine. b Comparison of the bone marrow cells harvested from three distinct anatomical regions: left leg, right leg, and spine. c Leukemia clonal diversity across different tissues and organs from one representative mouse. Additional mice are shown in Supplementary Fig. . Each color represents one distinct genetic barcode corresponding to a leukemia clone. d Comparison of clonal abundance between the peripheral blood and the spleen or the leg bone marrow for ALL04 clones. Markers of the same shape represent data from one mouse. 99% confidence intervals were determined by the blood and spleen comparison and highlighted by dashed lines. e Genes significantly differentially expressed in ALL04 clones more abundant in the BM compared to clones more abundant in the blood. The black bar indicates the mean, and the white dot represents the median. f , g Genes identified in ( e ) and a negative control luciferase ( LUC ) gene were knocked out using the CRISPR/Cas9 technology in B-ALL cell lines (REH, KOPN-8, and NALM6). f Adhesion of the B-ALL cells to OP9 stroma cells was analyzed after 48 h of co-incubation. Four independent experiments for REH ( n = 16 for LUC , <t>DNAJC</t> , LRIF ; n = 15 <t>for</t> <t>BTK</t> ). Three independent experiments for KOPN-8 ( n = 12 for LUC , BTK , LRIF ; n = 9 for DNAJC ). g Migration of the B-ALL cells was analyzed after 12 or 24 h of incubation (12 h for NALM6, 24 h for REH). Three independent experiments ( n = 12). f , g Data shown as mean ± SEM. ** P < 0.01, *** P < 0.001, by two-sided t test without adjustment. MNC mononuclear cells. BM bone marrow. Source data are provided as a Source Data file.
Crispick Online Tool, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispick online tool/product/Broad Institute Inc
Average 90 stars, based on 1 article reviews
crispick online tool - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
crispick sgrna design tool  (Broad Institute Inc)
90
Broad Institute Inc crispick sgrna design tool
a , b Heatmaps showing Pearson Correlations of clonal abundance across different tissues and organs. Each column corresponds to one primary recipient mouse. The clonal diversity of each mouse is shown below, where each color represents a unique clone. a Comparison between the peripheral blood, spleen, and bone marrow from the spine. b Comparison of the bone marrow cells harvested from three distinct anatomical regions: left leg, right leg, and spine. c Leukemia clonal diversity across different tissues and organs from one representative mouse. Additional mice are shown in Supplementary Fig. . Each color represents one distinct genetic barcode corresponding to a leukemia clone. d Comparison of clonal abundance between the peripheral blood and the spleen or the leg bone marrow for ALL04 clones. Markers of the same shape represent data from one mouse. 99% confidence intervals were determined by the blood and spleen comparison and highlighted by dashed lines. e Genes significantly differentially expressed in ALL04 clones more abundant in the BM compared to clones more abundant in the blood. The black bar indicates the mean, and the white dot represents the median. f , g Genes identified in ( e ) and a negative control luciferase ( LUC ) gene were knocked out using the CRISPR/Cas9 technology in B-ALL cell lines (REH, KOPN-8, and NALM6). f Adhesion of the B-ALL cells to OP9 stroma cells was analyzed after 48 h of co-incubation. Four independent experiments for REH ( n = 16 for LUC , <t>DNAJC</t> , LRIF ; n = 15 <t>for</t> <t>BTK</t> ). Three independent experiments for KOPN-8 ( n = 12 for LUC , BTK , LRIF ; n = 9 for DNAJC ). g Migration of the B-ALL cells was analyzed after 12 or 24 h of incubation (12 h for NALM6, 24 h for REH). Three independent experiments ( n = 12). f , g Data shown as mean ± SEM. ** P < 0.01, *** P < 0.001, by two-sided t test without adjustment. MNC mononuclear cells. BM bone marrow. Source data are provided as a Source Data file.
Crispick Sgrna Design Tool, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispick sgrna design tool/product/Broad Institute Inc
Average 90 stars, based on 1 article reviews
crispick sgrna design tool - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


a , b Heatmaps showing Pearson Correlations of clonal abundance across different tissues and organs. Each column corresponds to one primary recipient mouse. The clonal diversity of each mouse is shown below, where each color represents a unique clone. a Comparison between the peripheral blood, spleen, and bone marrow from the spine. b Comparison of the bone marrow cells harvested from three distinct anatomical regions: left leg, right leg, and spine. c Leukemia clonal diversity across different tissues and organs from one representative mouse. Additional mice are shown in Supplementary Fig. . Each color represents one distinct genetic barcode corresponding to a leukemia clone. d Comparison of clonal abundance between the peripheral blood and the spleen or the leg bone marrow for ALL04 clones. Markers of the same shape represent data from one mouse. 99% confidence intervals were determined by the blood and spleen comparison and highlighted by dashed lines. e Genes significantly differentially expressed in ALL04 clones more abundant in the BM compared to clones more abundant in the blood. The black bar indicates the mean, and the white dot represents the median. f , g Genes identified in ( e ) and a negative control luciferase ( LUC ) gene were knocked out using the CRISPR/Cas9 technology in B-ALL cell lines (REH, KOPN-8, and NALM6). f Adhesion of the B-ALL cells to OP9 stroma cells was analyzed after 48 h of co-incubation. Four independent experiments for REH ( n = 16 for LUC , DNAJC , LRIF ; n = 15 for BTK ). Three independent experiments for KOPN-8 ( n = 12 for LUC , BTK , LRIF ; n = 9 for DNAJC ). g Migration of the B-ALL cells was analyzed after 12 or 24 h of incubation (12 h for NALM6, 24 h for REH). Three independent experiments ( n = 12). f , g Data shown as mean ± SEM. ** P < 0.01, *** P < 0.001, by two-sided t test without adjustment. MNC mononuclear cells. BM bone marrow. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Deciphering intratumoral heterogeneity using integrated clonal tracking and single-cell transcriptome analyses

doi: 10.1038/s41467-021-26771-1

Figure Lengend Snippet: a , b Heatmaps showing Pearson Correlations of clonal abundance across different tissues and organs. Each column corresponds to one primary recipient mouse. The clonal diversity of each mouse is shown below, where each color represents a unique clone. a Comparison between the peripheral blood, spleen, and bone marrow from the spine. b Comparison of the bone marrow cells harvested from three distinct anatomical regions: left leg, right leg, and spine. c Leukemia clonal diversity across different tissues and organs from one representative mouse. Additional mice are shown in Supplementary Fig. . Each color represents one distinct genetic barcode corresponding to a leukemia clone. d Comparison of clonal abundance between the peripheral blood and the spleen or the leg bone marrow for ALL04 clones. Markers of the same shape represent data from one mouse. 99% confidence intervals were determined by the blood and spleen comparison and highlighted by dashed lines. e Genes significantly differentially expressed in ALL04 clones more abundant in the BM compared to clones more abundant in the blood. The black bar indicates the mean, and the white dot represents the median. f , g Genes identified in ( e ) and a negative control luciferase ( LUC ) gene were knocked out using the CRISPR/Cas9 technology in B-ALL cell lines (REH, KOPN-8, and NALM6). f Adhesion of the B-ALL cells to OP9 stroma cells was analyzed after 48 h of co-incubation. Four independent experiments for REH ( n = 16 for LUC , DNAJC , LRIF ; n = 15 for BTK ). Three independent experiments for KOPN-8 ( n = 12 for LUC , BTK , LRIF ; n = 9 for DNAJC ). g Migration of the B-ALL cells was analyzed after 12 or 24 h of incubation (12 h for NALM6, 24 h for REH). Three independent experiments ( n = 12). f , g Data shown as mean ± SEM. ** P < 0.01, *** P < 0.001, by two-sided t test without adjustment. MNC mononuclear cells. BM bone marrow. Source data are provided as a Source Data file.

Article Snippet: Single guide RNAs (sgRNAs) targeting BTK, LRIF, DNAJC , and S100A16 were designed using the Broad Institute CRISPick online tool.

Techniques: Comparison, Clone Assay, Negative Control, Luciferase, CRISPR, Incubation, Migration